Cell Medicine : A FREE, online open-access journal. See full text articles below.

Co-Editors: David Eve & Dr. Dragos Cretoiu

ISSN: 2155-1790



Aims & Scope

Cell Medicine informs society about original, peer-reviewed research and review articles on the subject of cell therapy and its translational application to human diseases. A wide range of topics involving the use of cells, engineered cells and stem cells derived from a multitude of different sources, covering aspects of physiological, medical, tissue engineering, and device-oriented manipulation of the nervous system, endocrine, cardiac, bone, skin, muscle and other organs, with the ultimate aim of clinical translation are acceptable. To provide complete coverage of this revolutionary field, Cell Medicine reports on relevant technological advances and their potential for translational medicine. Rigorous peer review is applied to all articles published in Cell Medicine to ensure only high quality contributions from all areas of translational research are accepted.

While the journal Cell Transplantation, issued by the same publishers, is primarily restricted to articles that directly relate to cell transplantation, Cell Medicine also encompasses other forms of cellular study that are expected to ultimately result in a translational therapy and so is not limited to cellular transplantation.

Cell Medicine is an online Open Access journal. This allows your work to be disseminated to a wider audience and also entitle you to a free PDF, as well as prepublication of an unedited version of your manuscript. 

Cell Medicine features: 

Original Contributions: Peer-reviewed, high-quality research investigations that represent new and significant contributions to translational science.

Review Articles: Reviews of major areas in cellular therapy. These may be of any length and are peer reviewed.

Brief Communications and Letters: Timely and brief peer-reviewed studies or reader’s comments on matters of interest or already published articles.

Submission Requirements: From the beginning of November 2009, authors are requested to submit the original manuscript (and revised manuscript if needed) via our ManuscriptCentralwebsite at http://mc.manuscriptcentral.com/cogcom-ct

Please include a cover letter, specifying your intent to submit to Cell Medicine, as well as containing the name, address, telephone, and fax number, and electronic mail address of the author responsible for correspondence.  Follow the General Form guidelines below to prepare the manuscript, figures, and tables.

At the time of submission you will be asked to confirm that you will pay the relatively inexpensive open access fees ($900 for less than 5 pages, $1800 for 5-12 pages and +$75 for each additional page) when billed. In addition, there are sections for detailing any conflicts of interest and financial support and that you (as corresponding/submitting author) have the permission of the other authors to submit the manuscript. You will be given the option of which section of the editorial office to submit to. Here you would select Cell Medicine.

There will also be a $105 submission fee.

On receipt of your manuscript, it will be checked to ensure that it is correctly formatted. Manuscripts are considered for publication with the understanding that they have not been published elsewhere except in abstract form and are not concurrently under review elsewhere.

The editorial office will then assign a section editor, who is an expert in their field, to handle the review process for the manuscript. Expert reviewers from all over the world will be solicited untila minimum of two have agreed to extensively review the manuscript. The section editor will then make a decision about the acceptability of the manuscript based on the reviewer’s comments.

When the manuscript is accepted for publication, the author(s) will be required to provide a clean electronic copy of the manuscript including any figures as image files at the appropriate resolution (see Final Accepted Manuscript and Figure Files sections below).

General Form: Papers should be typed in English, double spaced throughout with at least 3-cm margins on paper approximately 22 x 28 cm (8 1/2 x 11 in.) in size. Please consult the most recent issue of the journal for style and format (http://www.ingentaconnect.com/content/cog/cm). Number all pages consecutively, beginning with the title page. Use metric units of measure; other units may be given in parentheses. Typically, only three levels of headings are recognized. The manuscript should be organized as follows.

Title Page: The title should be brief and specific. The title page should contain in the following order: title, name(s) and affiliation(s) of author(s) including city, state, postal code, and country, and a suggested running head of not more than 50 characters and spaces. Also indicate the author to whom correspondence should be addressed, with complete mailing address, telephone and fax numbers, and e-mail (necessary). Recognition of support can be provided as an unnumbered footnote.

Abstract: An abstract of 300 words or less should begin on page 2. It should contain a concise summary of the results, conclusions, and other significant points in the review.

Key words: For the purpose of subject indexing, provide four to six key words immediately following the abstract.

Text: Every effort should be made to avoid jargon, to spell out all nonstandard abbreviations the first time they are used, and to present the contents of the study as clearly and concisely as possible. References should be given for all discussions and conclusions of previous studies. Trade names may appear in parentheses and should be capitalized. Critical discussions of the literature are preferred more than historically oriented information.

Acknowledgements and Disclosures: Please acknowledge any funding support. In addition, all authors need to disclose any conflicts of interest, financial or otherwise, in a separate section before the references. Conflicts of interest may include: 
1. Direct ownership of equity or shares in a health care or pharmaceutical company relating to the manuscript, by any author or their immediate family members. 
2. Receipt of any form of income by any author or their immediate family members from health care or pharmaceutical companies related to the manuscript within the calendar year preceding original submission. 
3. Personal interest such as being an expert witness, public advocate, grantee, consultant, founder, owner, or employee of a health care or pharmaceutical company related to the research.

Human Studies: For human subjects to be included in publications, informed consent and ethical approval from the appropriate institutional body confirming adherence to the Helsinki Declaration for the study is required. These patients have a right to privacy that should not be infringed without their informed consent, meaning that no identifying information that could jeopardize their privacy can be included without the patient's consent.
All animal work requires confirmation of approval by the appropriate institutional body for the study that the animals were handled appropriately in accordance with the National Institutes of Health "Guide for the Care and Use of Laboratory Animals".

See the International Committee of Medical Journal Editors (http://www.icmje.org/) "Uniform Requirements for Manuscripts Submitted to Biomedical Journals" for further information.

References: Literature cited should be prepared according to the Council of Science Editors format (citation-sequence system). This format is conveniently in Endnote and the output style is available at the following site:http://endnote.com/downloads/style/cse-style-manual-7th-ed-citation-sequence. Some examples are provided below. References in the text should be cited by superscript number separated by a comma and listed in numerical order as they appear in the text (double spaced) on a separate page at the end of the manuscript. Journal citations in the reference list should contain the following: (a) reference number in superscript; (b) surnames and initials of all authors (surnames precede initials); (c) title of article; (d) journal title abbreviated as listed in ISSN.org; (e) year; volume, inclusive pages. See the examples shown and refer to Council of Science Editors format for more examples.

Journal Article: Wang Y, Nathanson L, McNiece IK. Differential hematopoietic supportive potential and gene expression of stroma cell lines from midgestation mouse placenta and adult bone marrow. Cell Transplant. 2011;20:707–26.

Book Article/Chapter: Taylor MJ, Baicu, S. Hypothermic perfusion of pancreas: Emphasis on preservation prior to islet isolation. In: Uygun K, Lee CY, editors. Organ preservation and reengineering. Boston (MA): Artech House Publisher; 2011. p. 85-104.

Book: Wang-Fischer Y. Manual of stroke models in rats, 1st ed. Boca Raton (FL): CRC Press; 2009.

Internet Source: Stem Cell Research Oversight Committee. University of Wisconsin-Madison policy for multisite research studies using human pluripotent stem cells [Internet]. Madison (WI): University of Wisconsin-Madison; 2009 [cited 2013 Sep 12]. Available from http://www.grad.wisc.edu/admin/committees/scro/documents/MultisiteresearchpolicyFinal.pdf

An example of an in-text citation is shown below.

Traumatic life events and posttraumatic stress disorder (PTSD) are endemic among American civilians 1.

To cite multiple sources, all numbers associated with the reference being cited should be superscript, separated by a comma, with no spaces between them.

Tables: Tables should be numbered and cited sequentially in the text. Prepare each table as a separate page at the end of the manuscript text, after the references. Avoid very wide or long tables that would not fit a printed page. Each table should have a title, and each column in the table should have a brief heading. Define all abbreviations in the table footnote at the bottom of the table.

Figures: Figures should be numbered and cited sequentially in the text. Each figure should be provided as a separate file (see Figure Files). Include all parts of a figure (e.g., A, B, etc.) as one file. Do not use light lettering and shading that will not reproduce well. Figure dimensions and scaling should be suitable for reduction (if necessary) to fit column or page size. Care must be taken that letters and other symbols do not become so small that they are illegible when the figure is reduced. (Do not embed figures within the manuscript text. Prepare as separate files or at the end of the manuscript, after tables and figure legends.)

Figure Legends: List all figure legends sequentially on one or more pages at the end of the manuscript text, after the references, and identify all symbols used in the figures. The figure legend should be as clear as possible and should fully describe the contents of the figure. (Do not include the figure legend as part of the figure). If the figure is from a previously published article, indicate that permission has been obtained from the original publisher.

Figure Files: Each figure should be provided as a separate, high-resolution file. Simple black and white figures (e.g., line graphs, bar graphs, etc.) should be 1200 dpi. Halftone and color figures (or combo figures) should be 600 dpi. Final figure files should be submitted as tiff, jpg, or eps format. Do not include the figure number as part of the figure file (e.g., do not label Figure 1, etc., as part of the figure).

Permissions: If data from any other source is used in tables or figures it is the responsibility of the author(s) to obtain permission to reproduce such material. Provide proof that permission has been granted from the original publisher and indicate the source.

Final Accepted Manuscript/Disk: When the manuscript is accepted for publication, the author(s) must provide by e-mail/CD or via a file uploading site one clean electronic copy of the manuscript in Word format, and the figure files at the appropriate resolution and format (see Figure Files section above). The final manuscript file (including tables and figure legends) must be submitted in IBM-compatible form, either as a Word document or as a plain text (ASCII) file. A PDF file at this stage is not acceptable. Once the final manuscript is correctly formatted, an unedited version of the manuscript will go online. Later, the manuscript will be copyedited for journal publication.

Page Proofs and Offprints: All material accepted for publication is subject to copyediting. Authors will receive page proofs of articles before publication and should answer all queries and carefully check all editorial changes at this point. Any corrections to proofs must be restricted to printer's errors. Along with the page proofs, the corresponding author will receive a form for ordering reprints and full copies of the issue in which the article appears. All co-author reprint requirements should be included on the Offprint Order Form.

For any questions relating to the formatting or submitting of manuscripts please contact:

David Eve, Associate Editor 
E-mail:  This e-mail address is being protected from spambots. You need JavaScript enabled to view it
Tel:  813-974-6169 
Fax: 813-974-3078

The publishers and editorial board of Cell Medicine have adopted the publication ethics and malpractice statements of the Committee on Publication Ethics (COPE) (http://publicationethics.org/resources/guidelines). These guidelines highlight what is expected of authors and what they can expect from the reviewers and editorial board in return. They also provide details of how problems will be handled. Briefly:

Author Responsibilities:
Authors listed on a manuscript must have made a significant contribution to the study and/or writing of the manuscript. During revisions, authors cannot be removed without their permission and that of the other authors. All authors must also agree to the addition of new authors.  It is the responsibility of the corresponding author to ensure that this occurs.

Financial support and conflicts of interest for all authors must be declared.  Further information on this can be obtained from the International Committee for Medical Journal Editors (http://www.icmje.org/).

The reported research must be novel and authentic and the authors should confirm that the same data has not been and is not going to be submitted to another journal (unless already rejected).  Statements made in the introduction and discussion should be supported by appropriate references and sufficient experimental detail should be provided to allow for repetition of the study by another group. Plagiarism of the text/data will not be tolerated and could result in retraction of an accepted article. Any text or figures reproduced for another source require the permission of the original copyright holders (normally the publishers).

Any manipulation of figures should be equally applied and described in the text including pseudocoloring and must not change the meaning of the figure.

When humans, animals or tissue derived from them have been used, then mention of the appropriate ethical approval must be included in the manuscript.

Reviewer Responsibilities: Reviewers are expected to not possess any conflicts of interest with the authors and research. They should review the science objectively and provide recommendations for improvements where necessary. When aware of relevant published work not being cited, the reviewers should recommend inclusion of these references.  If the reviewer feels that they would be unable to repeat the study as described, then additional methodological details should be requested. Any unpublished information read by a reviewer should be treated as confidential.

Editorial Responsibilities: The section editors are expected to select an appropriate number of reviewers for the manuscript so that they can make an informed decision about whether to reject/accept a manuscript. Their decision must be based only on the paper’s importance, originality and clarity and whether it is suitable for the journal. They must not have a conflict of interest with the authors or work described. The anonymity of the reviewers must be maintained.

NIH Public Access Policy: Cognizant Communication Corporation does not upload manuscripts on the author’s behalf to PubMedCentral. The authors of NIH-funded manuscripts are granted permission to upload the final version of the manuscript themselves to PubMedCentral so that they remain in compliance with the NIH Public Access Policy. A PDF of the article is provided to the Corresponding Author for this purpose. Authors have the opportunity to download their articles from open access files http://www.ingentaconnect.com/content/cog/cm

Should problems come to light after acceptance then the editors agree to promote the publication of corrections and/or retractions as deemed necessary.

Publishing Responsibilities: The publishers agree to ensure that to the best of their abilities, the information that they publish is genuine and ethically sound. If publishing ethics issues come to light, not limited to accusations of fraudulent data or plagiarism, during or after the publication process, they will be investigated by the editorial board including contact with the author’s institutions if necessary, so that a decision on the appropriate corrections, clarifications or retractions can be made. The publishers agree to publish this as necessary so as to maintain the integrity of the academic record.

(Scroll down to view tables of contents for all Volumes and Issues)

Volume 9, Number 1

Facilitating Transplantation 1
Hirofumi Noguchi

Cell Therapy for Liver Disease Using Bioimaging Rats 3
Junko Haga, Shin Enosawa, and Eiji Kobayashi

Challenges for Production of Human Transplantable Organ Grafts 9
Eiji Kobayashi

Evaluation of Serum-Free, Xeno-Free Cryopreservation Solutions for Human Adipose-Derived Mesenchymal Stem Cells 15
Chika Miyagi-ShiohiraNaoya Kobayashi, Issei Saitoh, Masami Watanabe, Yasufumi Noguchi, Masayuki Matsushita, and Hirofumi Noguchi

Immunomodulatory Effects of Adipose Tissue-Derived Stem Cells on Concanavalin A-Induced Acute Liver Injury in Mice 21
Yasuma Yoshizumi, Hiroshi Yukawa, Ryoji Iwaki, Sanae FujinakaAyano Kanou, Yuki Kanou, Tatsuya Yamada, Shingo Nakagawa, Tomomi Ohara, Kenta Nakagiri, Yusuke Ogihara, Yoko TsutsuiYumi Hayashi, Masatoshi IshigamiYoshinobu Baba, and Tetsuya Ishikawa

Enhanced Adipogenic Differentiation of Human Adipose-Derived Stem Cells in an In Vitro Microenvironment: The Preparation of Adipose-Like Microtissues Using a Three-Dimensional Culture 35
Yoshitaka Miyamoto, Masashi Ikeuchi, Hirofumi Noguchi, Tohru Yagi, and Shuji Hayashi

The Evaluation of Islet Purification Methods That Use Large Bottles to Create a Continuous Density Gradient 45
Chika Miyagi-ShiohiraNaoya Kobayashi, Issei Saitoh, Masami Watanabe, Yasufumi Noguchi, Masayuki Matsushita, and Hirofumi Noguchi

Comparison of Purification Solutions With Different Osmolality for Porcine Islet Purification 53
Chika Miyagi-ShiohiraNaoya Kobayashi, Issei Saitoh, Masami Watanabe, Yasufumi Noguchi, Masayuki Matsushita, and Hirofumi Noguchi

Measurement of DNA Length Changes Upon CpG Hypermethylation by Microfluidic Molecular Stretching 61
Daisuke Onoshima, Naoko Kawakita, Daiki Takeshita, Hirohiko Niioka, Hiroshi Yukawa, Jun Miyake, and Yoshinobu Baba

Volume 8, Number 3

Allogeneic Mesenchymal Stem Cell Transplantation in Dogs With Keratoconjunctivitis Sicca 63
Maura K. W. Bittencourt, Michele A. Barros, João Flávio P. Martins, Jose Paulo C. Vasconcellos, Bruna P. Morais, Celine Pompeia, Matheus Domingues BittencourtKarine dos Santos Evangelho, Irina Kerkis, and Cristiane V. Wenceslau

αSMA Expression in Large Colonies of Colony-Forming Units-Fibroblast as an Early Predictor of Bone Marrow MSC Expandability 79
Irina Aizman, William S. Holland, Cher Yang, and Damien Bates

Stability Enhancement Using Hyaluronic Acid Gels for Delivery of Human Fetal Progenitor Tenocytes 87
A. Grognuz, C. Scaletta, A. Farron, D. P. Pioletti, W. Raffoul, and L. A. Applegate

Aggregation of Engineered Human β-Cells Into Pseudoislets: Insulin Secretion and Gene Expression Profile in Normoxic and Hypoxic Milieu 99
Marie-José LecomteSéverine Pechberty, Cécile Machado, Sandra Da Barroca, Philippe Ravassard, Raphaël Scharfmann, Paul Czernichow, and Bertrand Duvillié

Volume 8, Numbers 1–2

Creating a Future of Transplantation 1
Hirofumi Noguchi, Guest Editor-in-Chief, JSOPMB Issue

Cryopreservation of Adipose-Derived Mesenchymal Stem Cells 3
Chika Miyagi-Shiohira, Kiyoto KurimaNaoya Kobayashi, Issei Saitoh, Masami Watanabe, Yasufumi Noguchi, Masayuki Matsushita, and Hirofumi Noguchi

Choice of Feeders Is Important When First Establishing iPSCs Derived From Primarily Cultured Human Deciduous Tooth Dental Pulp Cells 9
Issei Saitoh, Emi Inada, Yoko Iwase, Hirofumi Noguchi, Tomoya Murakami, Miki Soda, Naoko Kubota, Hiroko Hasegawa, Eri Akasaka, Yuko Matsumoto, Kyoko Oka, Youichi Yamasaki, Haruaki Hayasaki, Masahiro Sato

Islet Culture/Preservation Before Islet Transplantation 25
Hirofumi Noguchi, Chika Miyagi-Shiohira, Kiyoto KurimaNaoya Kobayashi, Issei Saitoh, Masami Watanabe, Yasufumi Noguchi, and Masayuki Matsushita

Efficient Gene Transduction of Dispersed Islet Cells in Culture Using Fiber-Modified Adenoviral Vectors 31
Hiroyuki Hanayama, Kazuo OhashiRie Utoh, Hirofumi Shimizu, Kazuya IseFuminori Sakurai, Hiroyuki Mizuguchi, Hiroyuki Tsuchiya, Teruo Okano, and Mitsukazu Gotoh

Human Laminin Isotype Coating for Creating Islet Cell Sheets 39
Shingo Yamashita, Kazuo OhashiRie UtohTeruo Okano, and Masakazu Yamamoto

Spheroid Formation and Evaluation of Hepatic Cells in a Three-Dimensional Culture Device 47
Yoshitaka Miyamoto, Masashi Ikeuchi, Hirofumi Noguchi, Tohru Yagi, and Shuji Hayashi

Fluorescence Quenching of CdSe/ZnS Quantum Dots by Using Black Hole Quencher Molecules Intermediated With Peptide for Biosensing Application 57
Sreenadh Sasidharan Pillai, Hiroshi Yukawa, Daisuke OnoshimaVasudevanpillai Biju, and Yoshinobu Baba

Volume 7, Number 3

Immature Dental Pulp Stem Cells Showed Renotropic and Pericyte-Like Properties in Acute Renal Failure in Rats 95
Michele A. Barros, Joao Flavio Panattoni Martins, Durvanei Augusto Maria, Crisitiane Valverde Wenceslau, Dener Madeiro De Souza, Alexandre Kerkis, Niels Olsen S. Camara, Julio Cesar C. Balieiro, and Irina Kerkis

A Rotating Bioreactor for Scalable Culture and Differentiation of Respiratory Epithelium 109

Micha Sam Brickman Raredon, Mahboobe Ghaedi, Elizabeth A. Calle, and Laura E. Niklason

A Combination of Low-Intensity Pulsed Ultrasound and Nanohydroxyapatite Concordantly Enhances Osteogenesis of Adipose-Derived Stem Cells From Buccal Fat Pad 123
Rika Nagasaki, Yoshiki Mukudai, Yasumasa Yoshizawa, Masahiro Nagasaki, Sunao Shiogama, Maiko Suzuki, Seiji Kondo, Satoru Shintani, and Tatsuo Shirota


Disease and Stem Cell-Based Analysis of the 2014 ASNTR Meeting 133
David J. Eve

Volume 7, Number 2


Basic and Clinical Science for Organ Biology

Hirofumi Noguchi, Guest Editor-in-Chief, JSOPMB Issue

Synergistic Effects of Calcineurin Inhibitors and Steroids on Steroid Sensitivity of Peripheral Blood Mononuclear Cells            
Hironori Takeuchi, Hitoshi Iwamoto, Yuki Nakamura, Toshihiko Hirano, Osamu Konno, Yu Kihara, Naokazu Chiba, Takayoshi Yokoyama, Kiminori Takano, Tatsunori Toraishi, Kiyoshi Okuyama, Chie Ikeda, Sachiko Tanaka, Kenji Onda, Akiko Soga, Yukiko Kikuchi, Takashi Kawaguchi, Shigeyuki Kawachi, Sakae Unezaki, and Motohide Shimazu

Improvement of Infusion Process in Cell Transplantation: Effect of Shear Stress on Hepatocyte Viability Under Horizontal and Vertical Syringe Orientation

Sandi Sufiandi, Hiromichi Obara, Shin Enosawa, Huai-Che Hsu, Naoto Matsuno, and Hiroshi Mizunuma

Three-Dimensional In Vitro Hepatic Constructs Formed Using Combinatorial Tapered Stencil for Cluster Culture (TASCL) Device

Yoshitaka Miyamoto, Masashi Ikeuchi, Hirofumi Noguchi, TohruYagi, and Shuji Hayashi

Influence of Autofluorescence Derived From Living Body on In Vivo Fluorescence Imaging Using Quantum Dots

Hiroshi Yukawa, Masaki Watanabe, Noritada Kaji, and Yoshinobu Baba

Potential Factors for the Differentiation of ESCs/iPSCs Into Insulin-Producing Cells

Takako Tsugata, Naruo Nikoh, Tatsuya Kin, Issei Saitoh, Yasufumi Noguchi, Hideo Ueki, Masami Watanabe, Andrew M. James Shapiro, and Hirofumi Noguchi

Volume 7, Number 1


Application of Induced Pluripotent Stem Cells in Liver Diseases 1
Yue Yu, Xuehao Wang, and Scott L. Nyberg

Original Contribution

Integration-Free Human Induced Pluripotent Stem Cells From Type 1 Diabetes Patient Skin Fibroblasts Show Increased Abundance of Pancreas-Specific microRNAs 15
Jun Liu, Mugdha V. Joglekar, Huseyin Sumer, Anandwardhan A. Hardikar, Halena Teede, and Paul J. Verma

Human Menstrual Blood-Derived Mesenchymal Cells as New Human Feeder Layer System for Human Embryonic Stem Cells 25
Danubia Silva dos Santos, Vanessa Carvalho Coelho de Oliveira, Karina Dutra Asensi, Leandro Vairo, Adriana Bastos Carvalho, Antonio Carlos Campos de Carvalho, and Regina Coeli dos Santos Goldenberg

Preculturing Islets With Adipose-Derived Mesenchymal Stromal Cells Is an Effective Strategy for Improving Transplantation Efficiency at the Clinically Preferred Intraportal Site
Chloe L. Rackham, Paramjeet K. Dhadda, Aurelie M. Le Lay, Aileen J. F. King, and Peter M. Jones

Volume 6, Number 3

Original Contribution

Autologous Skeletal Myoblast Sheet Therapy for Porcine Myocardial Infarction Without Increasing Risk of Arrhythmia
Yutaka Terajima, Tatsuya Shimizu, Shinpei Tsuruyama, Hidekazu Sekine, Hikaru Ishii, Kenji Yamazaki, Nobuhisa Hagiwara, and Teruo Okano


Estrogen Replacement Therapy for Stroke
Mibel Pabon, Cyrus Tamboli, Sarosh Tamboli, Sandra Acosta, Ike De La Pena, Paul R. Sanberg, Naoki Tajiri, Yuji Kaneko, and Cesar V. Borlongan

Oligodendrocytes Engineered With Migratory Proteins as Effective Graft Source for Cell Transplantation in Multiple Sclerosis
Ike De La Pena, Mibel Pabon, Sandra Acosta, Paul R. Sanberg, Naoki Tajiri, Yuji Kaneko, and Cesar V. Borlongan

Disease and Stem Cell-Based Analysis of the 2013 ASNTR Meeting
David J. Eve

Volume 6, Numbers 1–2

Organ Biology—New Development
Hirofumi Noguchi, Guest Editor in Chief, JSOPMB Issue

Comparison of New Preservation Solutions, HN-1 and University of Wisconsin Solution, in Pancreas Preservation for Porcine Islet Isolation
Akihiro Katayama, Hirofumi Noguchi, Takashi Kuise, Atsuko Nakatsuka, Daisho Hirota, Hitomi Usui Kataoka, Takashi Kawai, Kentaro Inoue, Noriko Imagawa, Issei Saitoh, Yasufumi Noguchi, Masami Watanabe, Jun Wada, and Toshiyoshi Fujiwara

Comparison of Incubation Solutions Prior to the Purification of Porcine Islet Cells
Takashi Kawai, Hirofumi Noguchi, Takashi Kuise, Atsuko Nakatsuka, Akihiro Katayama, Noriko Imagawa, Hitomi Usui Kataoka, Issei Saitoh, Yasufumi Noguchi, Masami Watanabe, and Toshiyoshi Fujiwara

Maintenance of Viability and Function of Rat Islets With the Use of ROCK Inhibitor Y-27632
Yasuhiro Kubota, Hirofumi Noguchi, Masayuki Seita, Takeshi Yuasa, Hiromi Sasamoto, Shuhei Nakaji, Teru Okitsu, Toshiyoshi Fujiwara, and Naoya Kobayashi

Development of Canine Models of Type 1 Diabetes With Partial Pancreatectomy and the Administration of Streptozotocin
Masayuki Seita, Hirofumi Noguchi, Yasuhiro Kubota, Hironobu Kawamoto, Shuhei Nakaji, Naoya Kobayashi, and Toshiyoshi Fujiwara

Quality of Air-Transported Human Islets for Single Islet Cell Preparations
Shingo Yamashita, Kazuo Ohashi, Rie Utoh, Tatsuya Kin, A. M. James Shapiro, Masakazu Yamamoto, Mitsukazu Gotoh, and Teruo Okano

Comparison of the Pharmacological Efficacies of Immunosuppressive Drugs Evaluated by the ATP Production and Mitochondrial Activity in Human Lymphocytes
Hiroyasu Sasahara, Kentaro Sugiyama, Mahoto Tsukaguchi, Kazuya Isogai, Akira Toyama, Hiroshi Satoh, Kazuhide Saitoh, Yuki Nakagawa, Kota Takahashi, Sachiko Tanaka, Kenji Onda, and Toshihiko Hirano

Peripheral Lymphocyte Response to Mycophenolic Acid In Vitro and Incidence of Cytomegalovirus Infection in Renal Transplantation
Kentaro Sugiyama, Hiroyasu Sasahara, Mahoto Tsukaguchi, Kazuya Isogai, Akira Toyama, Hiroshi Satoh, Kazuhide Saitoh, Yuki Nakagawa, Kota Takahashi, Sachiko Tanaka, Kenji Onda, and Toshihiko Hirano

Experimental Nonalcoholic Steatohepatitis Induced by Neonatal Streptozotocin Injection and a High-Fat Diet in Rats
Huai-Che Hsu, Masaharu Dozen, Naoto Matsuno, Hiromichi Obara, Ryou Tanaka, and Shin Enosawa

Electron Therapy Attenuated Elevated Alanine Aminotransferase and Oxidative Stress Values in Type 2 Diabetes-Induced Nonalcoholic Steatohepatitis of Rats
Shin Enosawa, Masaharu Dozen, Yuki Tada, and Keisuke Hirasawa

STO Feeder Cells Are Useful for Propagation of Primarily Cultured Human Deciduous Dental Pulp Cells by Eliminating Contaminating Bacteria and Promoting Cellular Outgrowth
Tomoya Murakami, Issei Saitoh, Emi Inada, Mie Kurosawa, Yoko Iwase, Hirofumi Noguchi, Yutaka Terao, Youichi Yamasaki, Haruaki Hayasaki, and Masahiro Sato

Induced Pluripotent Stem Cell Labeling Using Quantum Dots
Hiroshi Yukawa, Kaoru Suzuki, Yuki Kano, Tatsuya Yamada, Noritada Kaji, Tetsuya Ishikawa, and Yoshinobu Baba

Adipose Tissue-Derived Stem Cell Imaging Using Cadmium-Free Quantum Dots
Yoshiyuki Miyazaki, Hiroshi Yukawa, Hiroyasu Nishi, Yukihiro Okamoto, Noritada Kaji, Tsukasa Torimoto, and Yoshinobu Baba

Volume 5, Numbers 2-3

Japan Society for Organ Preservation and Medical Biology (JSOPMB)

Long-Expected New Start
Hirofumi Noguchi, Guest Editor-in-Chief, JSOPMB Issue


Bioimaging of Transgenic Rats Established at Jichi Medical University: Applications in Transplantation Research
Takumi Teratani and Eiji Kobayashi

ER Stress and b-Cell Pathogenesis of Type 1 and Type 2 Diabetes and Islet Transplantation
Hitomi Usui Kataoka and Hirofumi Noguchi

A Review of Autologous Islet Transplantation
Michihiro Maruyama, Takashi Kenmochi, Naotake Akutsu, Kazunori Otsuki, Taihei Ito, Ikuko Matsumoto, and Takehide Asano

Original Contributions

Culture Conditions for Mouse Pancreatic Stem Cells
Hirofumi Noguchi, Issei Saitoh, Hitomi Usui Kataoka, Masami Watanabe, Yasufumi Noguchi, and Toshiyoshi Fujiwara

Isolation Efficiency of Mouse Pancreatic Stem Cells Is Age Dependent
Takashi Kuise, Hirofumi Noguchi, Issei Saitoh, Hitomi Usui Kataoka, Masami Watanabe, Yasufumi Noguchi, and Toshiyoshi Fujiwara

Use of Mesenchymal Stem Cell-Conditioned Medium to Activate Islets in Preservation Solution
Naoya Kasahara, Takumi Teratani, Junshi Doi, Yuki Iijima, Masashi Maeda, Shinji Uemoto, Yasuhiro Fujimoto, Naohiro Sata, Yoshikazu Yasuda, and Eiji Kobayashi

Inhibition of Hepatic Ischemic Reperfusion Injury Using Saline Exposed to Electron Discharge in a Rat Model
Masaharu Dozen, Shin Enosawa, Yuki Tada, and Keisuke Hirasawa

Observation of Positively Charged Magnetic Nanoparticles Inside HepG2 Spheroids Using Electron Microscopy
Yoshitaka Miyamoto, Yumie Koshidaka, Hirofumi Noguchi, Koichi Oishi, Hiroaki Saito, Hiroshi Yukawa, Noritada Kaji, Takeshi Ikeya, Satoshi Suzuki, Hisashi Iwata, Yoshinobu Baba, Katsutoshi Murase, and Shuji Hayashi

Improved Recovery of Hepatocytes Isolated From Warm Ischemic Rat Liver by Citrate Phosphate Dextrose (CPD)-Supplemented Euro-Collins Solution
Huai-Che Hsu, Naoto Matsuno, Noboru Machida, and Shin Enosawa

Volume 5, Number 1


Human Liver Progenitor Cells for Liver Repair
Catherine A. Lombard, Julie Prigent, and Etienne M. Sokal

Original Contributions

Transplantation of Human Adipose Tissue-Derived Mesenchymal Stem Cells Restores the Neurobehavioral Disorders of Rats With Neonatal Hypoxic-Ischemic Encephalopathy
Dongsun Park, Sun Hee Lee, Dae Kwon Bae, Yun-Hui Yang, Goeun Yang, Jangbeen Kyung, Dajeong Kim, Ehn-Kyoung Choi, Jin Tae Hong, Il Seob Shin, Sung Keun Kang, Jeong Chan Ra, and Yun-Bae Kim

Phenotype and Stability of Neural Differentiation of Androgenetic Murine ES Cell-Derived Neural Progenitor Cells
Wanja Wolber, Ruhel Ahmad, Soon Won Choi, Sigrid Eckardt, K. John McLaughlin, Jessica Schmitt, Christian Geis, Manfred Heckmann, Anna-Leena Siren, and Albrecht M. Muller

Volume 4, Number 3

Simple Machine Perfusion Significantly Enhances Hepatocyte Yields of Ischemic and Fresh Rat Livers
Maria-Louisa Izamis, Candice Calhoun, Basak E. Uygun, Maria Angela Guzzardi, Gavrielle Price, Martha Luitje, Nima Saeidi, Martin L. Yarmush, and Korkut Uygun

Human Decidua-Derived Mesenchymal Cells Are a Promising Source for the Generation and Cell Banking of Human Induced Pluripotent Stem Cells
Tomoko Shofuda, Daisuke Kanematsu, Hayato Fukusumi, Atsuyo Yamamoto, Yohei Bamba, Sumiko Yoshitatsu, Hiroshi Suemizu, Masato Nakamura, Yoshikazu Sugimoto, Miho Kusuda Furue, Arihiro Kohara, Wado Akamatsu, Yohei Okada, Hideyuki Okano, Mami Yamasaki, and Yonehiro Kanemura

Brief Communication

Cell Persistence of Allogeneic Keratinocytes and Fibroblasts Applied in a Fibrin Matrix to Acute, Full Thickness Wounds
Jaime E. Dickerson Jr., John V. Planz, Barry T. Reece, Kathy A. Weedon, Sandy D. Kirkpatrick, and Herbert B. Slade

Volume 4, Number 2

Nestin Overexpression Precedes Caspase-3 Upregulation in Rats Exposed to Controlled Cortical Impact Traumatic Brain Injury
Yuji Kaneko, Naoki Tajiri, SeongJin Yu, Takuro Hayashi, Christine E. Stahl, Eunkyung Bae, Humberto Mestre, Nicholas Franzese, Antonio Rodrigues Jr., Maria C. Rodrigues, Hiroto Ishikawa, Kazutaka Shinozuka, Whitney Hethorn, Nathan Weinbren, Loren E. Glover, Jun Tan, Anilkumar Harapanahalli Achyuta, Harry van Loveren, Paul R. Sanberg, Sundaram Shivsankar, and Cesar V. Borlongan

The Effect of CXCR4 Overexpression on Mesenchymal Stem Cell Transplantation in Ischemic Stroke
Oh Young Bang, Kyung Sil Jin, Mi Na Hwang, Ho Young Kang, Byoung Joon Kim, Sang Jin Lee, Sangmee Kang, Yu Kyeong Hwang, Jong Seong Ahn, and Ki Woong Sung

Use of Magnetocapsules for In Vivo Visualization and Enhanced Survival of Xenogeneic HepG2 Cell Transplants
Thomas W. Link, Dian R. Arifin, Christopher M. Long, Piotr Walczak, Naser Muja, Aravind Arepally, and Jeff W. M. Bulte

Improved Hepatocyte Engraftment After Portal Vein Occlusion in LDL Receptor-Deficient WHHL Rabbits and Lentiviral-Mediated Phenotypic Correction In Vitro
Sylvie Goulinet-Mainot, Hadrien Tranchart, Marie-Thérèse Groyer-Picard, Panagiotis Lainas, Papa Saloum Diop, Delphine Holopherne, Patrick Gonin, Karim Benihoud, Nathalie Ba, Olivier Gauthier, Dominique Franco, Catherine Guettier, Danièle Pariente, Anne Weber, Ibrahim Dagher, and Tuan Huy Nguyen

Behavior of Human Articular Chondrocytes During In Vivo Culture in Closed, Permeable Chambers
Iñigo Martinez-Zubiaurre, Tuija Annala, and Martin Polacek

Volume 4, Number 1

Comparative Analysis of the Immunomodulatory Properties of Equine Adult-Derived Mesenchymal Stem Cells
Danielle D. Carrade, Michael W. Lame, Michael S. Kent, Kaitlin C. Clark, Naomi J. Walker, and Dori L. Borjesson

Comparison of Gingiva, Dental Pulp, and Periodontal Ligament Cells From the Standpoint of Mesenchymal Stem Cell Properties
Koji Otabe, Takeshi Muneta, Nobuyuki Kawashima, Hideaki Suda, Kunikazu Tsuji, and Ichiro Sekiya

Epiphyseal Chondroprogenitors Provide a Stable Cell Source for Cartilage Cell Therapy
Salim Darwiche, Corinne Scaletta, Wassim Raffoul, Dominique P. Pioletti, and Lee Ann Applegate

Bioartificial Renal Epithelial Cell System (BRECS): A Compact, Cryopreservable Extracorporeal Renal Replacement Device
Deborah A. Buffington, Christopher J. Pino, Lijun Chen, Angela J. Westover, Gretchen Hageman, and H. David Humes

Adequate Time Window and Environmental Factors Supporting Retinal Graft Cell Survival in rd Mice
Michiko Mandai, Kohei Homma, Satoshi Okamoto, Chikako Yamada, Akane Nomori, and Masayo Takahashi


Organ/Cell Transplantation and Regenerative Medicine
Hirofumi Noguchi, Guest Editor-in-Chief, JSOPMB Issue

In Vivo Bioimaging Rats for Translational Research in Cell and Tissue Transplantation
Takumi Teratani and Eiji Kobayashi

Consideration of a Safe Protocol for Hepatocyte Transplantation Using Infantile Pigs
Shin Enosawa, Wenji Yuan, Masaharu Douzen, Atsuko Nakazawa, Takeshi Omasa, Akinari Fukuda, Seisuke Sakamoto, Takanobu Shigeta, and Mureo Kasahara

Construction of Artificial Hepatic Lobule-Like Spheroids on a Three-Dimensional Culture Device
Shin Enosawa, Yoshitaka Miyamoto, Hisayo Kubota, Tomoko Jomura, and Takeshi Ikeya

Hepatocyte Is a Sole Cell Type Responsible for the Production of Coagulation Factor IX In Vivo
Kohei Tatsumi, Kazuo Ohashi, Shigeki Mukobata, Atsushi Kubo, Fumikazu Koyama, Yoshiyuki Nakajima, Midori Shima, and Teruo Okano

A Combined Continuous Density/Osmolality Gradient for Supplemental Purification of Human Islets
Hirofumi Noguchi, Bashoo Naziruddin, Masayuki Shimoda, Daisuke Chujo, Morihito Takita, Koji Sugimoto, Takeshi Itoh, Nicholas Onaca, Marlon F. Levy, and Shinichi Matsumoto

Novel Positive-Charged Nanoparticles for Efficient Magnetic Resonance Imaging of Islet Transplantation
Koichi Oishi, Hirofumi Noguchi, Hiroaki Saito, Hiroshi Yukawa, Yoshitaka Miyamoto, Kenji Ono, Katsutoshi Murase, Makoto Sawada, and Shuji Hayashi

Differentiation of Mouse Pancreatic Stem Cells Into Insulin-Producing Cells by Recombinant Sendai Virus-Mediated Gene Transfer Technology
Hiroshi Yukawa, Hirofumi Noguchi, Koichi Oishi, Yoshitaka Miyamoto, Makoto Inoue, Mamoru Hasegawa, Shuji Hayashi, and Yoshinobu Baba

Eicosapentenoic Acid Attenuates Allograft Rejection in an HLA-B27/EGFP Transgenic Rat Cardiac Transplantation Model
Zhong Liu, Naoyuki Hatayama, Lin Xie, Ken Kato, Ping Zhu, Takahiro Ochiya, Yukitoshi Nagahara, Xiang Hu, and Xiao-Kang Li

Higher Sensitivity of Peripheral Blood Lymphocytes to Endogenous Glucocorticoid in Renal Transplant Recipients Treated With Tacrolimus, as Compared to Those Treated With Cyclosporine
Gulimire Muhetaer, Hironori Takeuchi, Sogo Akizuki, Hitoshi Iwamoto, Motohide Shimazu, Sakae Unezaki, and Toshihiko Hirano

Clinical Significance of the Pharmacological Efficacy of Tacrolimus Estimated by the Lymphocyte Immunosuppressant Sensitivity Test (LIST) Before and After Renal Transplantation
Kentaro Sugiyama, Kazuya Isogai, Akira Toyama, Hiroshi Satoh, Kazuhide Saito, Yuki Nakagawa, Masayuki Tasaki, Kota Takahashi, and Toshihiko Hirano

Cryopreservation of Induced Pluripotent Stem Cells
Yoshitaka Miyamoto, Hirofumi Noguchi, Hiroshi Yukawa, Koichi Oishi, Kenji Matsushita, Hisashi Iwata, and Shuji Hayashi

Generation of Mouse STO Feeder Cell Lines That Confer Resistance to Several Types of Selective Drugs
Issei Saitoh, Masahiro Sato, Yoko Iwase, Emi Inada, Toshiki Nomura, Eri Akasaka, Youichi Yamasaki, and Hirofumi Noguchi

A Simplified In Vitro Teratoma Assay for Pluripotent Stem Cells Injected Into Rodent Fetal Organs
Shigeo Masuda, Takashi Yokoo, Naomi Sugimoto, Masako Doi, Shuh-hei Fujishiro, Kengo Takeuchi, Eiji Kobayashi, and Yutaka Hanazono

Cell Therapy Using Adipose-Derived Stem Cells for Chronic Liver Injury in Mice
Kazuo Ohashi, Yoshinori Matsubara, Kohei Tatsumi, Ayako Kohori, Rie Utoh, Hiroshi Kakidachi, Akihiro Horii, Masahiro Tsutsumi, and Teruo Okano

Isolation of Hepatic Progenitor Cells From Human Liver With Cirrhosis Secondary to Biliary Atresia Using EpCAM or Thy-1 Markers
Taisuke Yamazaki, Shin Enosawa, Mureo Kasahara, Akinari Fukuda, Seisuke Sakamoto, Takanobu Shigeta, Atsuko Nakazawa, and Takayoshi Tokiwa

Side Population Cells From an Immortalized Human Liver Epithelial Cell Line Exhibit Hepatic Stem-Like Cell Properties
Takayoshi Tokiwa, Taisuke Yamazaki, and Shin Enosawa


Resident Endothelial Progenitor Cells From Human Placenta Have Greater Vasculogenic Potential Than Circulating Endothelial Progenitor Cells From Umbilical Cord Blood
Brian M. Rapp, M. Reza Saadatzedeh, Richard H. Ofstein, Janak R. Bhavsar, Zachary S. Tempel, Oscar Moreno, Peter Morone, Dana A. Booth, Dmitry O. Traktuev, Michael C. Dalsing, David A. Ingram, Mervin C. Yoder, Keith L. March, and Michael P. Murphy

Validation of Islet Transport From a Geographically Distant Isolation Center Enabling Equitable Access and National Health Service Funding of a Clinical Islet Transplant Program for England
Ali Aldibbiat, Guo Cai Huang, Min Zhao, Graham N. Holliman, Linda Ferguson, Stephen Hughes, Ken Brigham, Julie Wardle, Rob Williams, Anne Dickinson, Steven A. White, Paul R. V. Johnson, Derek Manas, Stephanie A. Amiel, and James A. M. Shaw

Brief Communication

Hypothermic Perfusion Preservation of Pancreas for Islet Grafts: Validation Using a Split Lobe Porcine Model
B. P. Weegman, M. J. Taylor, S. C. Baicu, W. E. Scott, III, K. R. Mueller, J. D. Kitzmann, M. D. Rizzari, and K. K. Papas



Mesenchymal Stromal Cells as a Therapeutic Strategy to Support Islet Transplantation in Type 1 Diabetes Mellitus
Sarah A. Busch, Saskia T. J. van Crutchen, Robert J. Deans, and Anthony E. Ting

Original Contributions

Early Immunomodulation by Intravenously Transplanted Mesenchymal Stem Cells Promotes Functional Recovery in Spinal Cord Injured Rats
Jung Hwa Seo, In Keun Jang, Hyongbum Kim, Mal Sook Yang, Jong Eun Lee, Hyo Eun Kim, Yong-Woo Eom, Doo-Hoon Lee, Ji Hea Yu, Ji Yeon Kim, Hyun Ok Kim, and Sung-Rae Cho

Neuroprotective and Angiogenic Effects of Bone Marrow Transplantation Combined With Granulocyte Colony-Stimulating Factor in a Mouse Model of Amyotrophic Lateral Sclerosis
Yasuyuki Ohta, Makiko Nagai, Kazunori Miyazaki, Nobuhito Tanaka, Hiromi Kawai, Takafumi Mimoto, Nobutoshi Morimoto, Tomoko Kurata, Yoshio Ikeda, Tohru Matsuura, and Koji Abe

Volume 2, Supplement 1

Cell Transplant Track Abstracts from the Cell Transplant Society - International Xenotransplantation Association 2011 Joint International Congress

Author Index for Cell Transplant Track Abstracts from the Cell Transplant Society - International Xenotransplantation Association 2011 Joint International Congress

Volume 2, Number 1


Prospects for Induced Pluripotent Stem Cell-Derived Hepatocytes in Cell Therapy
Masaya Iwamuro, Javed M. Shahid, Kazuhide Yamamoto, and Naoya Kobayashi

Management of Liver Failure: From Transplantation to Cell-Based Therapy
Maria Giovanna Francipane, Melchiorre Cervello, Giovanni Battista Vizzini, Giada Pietrosi, and Giuseppe Montalto

Original Contribution

Upregulation of Adipogenesis and Chondrogenesis in MSC Serum-Free Culture
Saey Tuan Barnabas Ho, Vivek Madhukar Tanavde, James Hoi Hui, and Eng Hin Lee

Volume 1, Number 3


Tracking Cells Without Leaving a Trace
Stephen Strom

Original Contributions

Autografting of Renal Progenitor Cells Ameliorates Kidney Damage in Experimental Model of Pyelonephritis
Abdol-Mohammad Kajbafzadeh, Azadeh Elmi, Saman Shafaat Talab, Zhina Sadeghi, Hamed Emami, and Masoud Sotoudeh

Monitoring of Liver Cell Transplantation in a Preclinical Swine Model Using Magnetic Resonance Imaging
Nathanael Raschzok, Ulf Teichgräber, Nils Billecke, Anja Zielinski, Kirsten Steinz, Nora N. Kammer, Mehmet H. Morgul, Sarah Schmeisser, Michaela K. Adonopoulou, Lars Morawietz, Bernhard Hiebl, Ruth Schwartlander, Wolfgang Rüdinger, Bernd Hamm, Peter Neuhaus, and Igor M. Sauer

Acute Treatment With Herbal Extracts Provides Neuroprotective Benefits in In Vitro and In Vivo Stroke Models, Characterized by Reduced Ischemic Cell Death and Maintenance of Motor and Neurological Functions
Yuji Kaneko, David J. Eve, SeongJin Yu, Hideki Shojo, Eunkyung Cate Bae, Dong-Hyuk Park, Bill Roschek, Jr., Randall S. Alberte, Paul R. Sanberg, Cyndy D. Sanberg, Paula C. Bickford, and Cesar V. Borlongan

Volume 1, Number 2

Original Contributions

Intracerebroventricular Transplantation of Cord Blood-Derived Neural Progenitors in a Child With Severe Global Brain Ischemic Injury
Sergiusz Jozwiak, Aleksandra Habich, Katarzyna Kotulska, Anna Sarnowska, Tomasz Kropiwnicki, Miroslaw Janowski, Elzbieta Jurkiewicz, Barbara Lukomska, Tomasz Kmiec, Jerzy Walecki, Marcin Roszkowski, Mieczyslaw Litwin, Tomasz Oldak, Dariusz Boruczkowski, and Krystyna Domanska-Janik

Transplant of Primary Human Hepatocytes Cocultured With Bone Marrow Stromal Cells to SCID Alb-uPA Mice
S. A. Mohajerani, M. Nourbakhsh, A. Cadili, J. R. Lakey, and N. M. Kneteman

In Vivo Growing of New Cell Colonies in a Portion of Bone Marrow: Potential Use for Indirect Cell Therapy
Ana Manzanedo, Fidel Rodriguez, Jose A. Obeso, and Manuel Rodriguez

Effects of Quantum Dot Labeling on Endothelial Progenitor Cell Function and Viability
Matyas Molnar, Peter Friberg, Ying Fu, Mikeal Brisslert, Michael Adams, and Yun Chen

Volume 1, Number 1


David Eve
Department of Neurosurgery & Brain Repair, MDC-78
University of South Florida Morsani College of Medicine
12901 Bruce B. Downs Blvd.
Tampa, FL 33612, USA

Dr. Dragos Cretoiu
Carol Davila University of Medicine and Pharmacy
Dept. of Cellular and Molecular Biology and Histology
8 Eroii Sanotari Blvd.
Bucharest 050474 Romania

Associate Editor: Samantha Portis, M.S.


Koji Abe, Okayama University, Japan
Rodolfo Alejandro, University of Miami School of Medicine, USA
Federico Bertuzzi, Ospedale Niguarda Cà Granda, Italy
Kenneth L. Brayman, University of Virginia Health System, USA
Enio Buffolo, Federal University of São Paulo, Brazil
Tom Chase, KM Pharmaceutical Consulting, Washington, DC
Fu-Chou Cheng, Taichung Veterans General Hospital, Taiwan
Tzyy-Wen Chiou, National Dong Hwa Univeristy, Taiwan
Michel Chopp, Henry Ford Hospital, USA
Francesca Cicchetti, CHUL, Canada
L. Eduardo Cruz, Cryopraxis, São Paulo, Brazil
Robert Deans, Athersys, Cleveland, OH
Anil Dhawan, King's College Hospital, UK
Wei-Ming Duan, Capital Medical University, China
Stephen B. Dunnett, Cardiff University, UK
Luis Fernandez, H4/782 Clinical Science Center, USA
Thomas B. Freeman, University of South Florida, USA
Ru-Huei Fu, China Medical University, Taiwan
Craig R. Halberstadt, Tengion, Inc., USA
Joshua Hare, University of Miami, USA
Horng-Jyh Harn, Chinal Medical University Hospital, Taiwan
Nelson A. Hossne Jr., Federal University of São Paulo, Brazil
Hongyun Huang, Beijing Hongtianji Neuroscience Academy, China
Johnny Huard, Rangos Research Center, USA
Dixon B. Kaufman, Northwestern University Medical School, USA
Jonathan Lakey, University of California, USA
Jian Lin, XBiotech, Austin, TX
Shinn-Zong Lin, China Medical University Hospital, Taiwan
Gele Liu, Carleton University, Canada
Shih-Ping Liu, China Medical University, Taiwan
Walter Low, University of Minnesota, USA
Daniela Moraes, University of São Paulo, Brazil
Keita Mori, SanBio, Mountain Valley, CA
Guido Nikkhah, Albert-Ludwigs-University, Germany
Andreas Nussler, University of Tübingen, Germany
Kazuo Ohashi, Tokyo Women's Medical University, Japan
Michael Ott, Hannover Medical School, Germany
Amit Patel, University of Utah, USA
Marc Penn, Cleveland Clinic Foundation, USA
Camillo Ricordi, University of Miami, USA
Maria C. O. Rodrigues, University of São Paulo, Brazil
Warren Sherman, Columbia University, USA
John Sinden, ReNeuron, Guildford, UK
John R. Sladek, University of Colorado, USA
Evan Y. Snyder, Burnham Institute for Medical Research, USA
Kwok-Fai So, University of Hong Kong, Hong Kong
Etienne M. Sokal, Université Catholique de Louvain, Belgium
Myron Spector, VA Boston Healthcare System, USA
Christof Stamm, Deutsches Herzzentrum Berlin, Germany
Stephen Strom, Karolinska Institute, Sweden
Hong-Lin Su, National Chung Hsing University, Taiwan
Jacques P. Tremblay, CHUL, Canada
Wise Young, Rutgers State of New Jersery, USA
Andrew Zeitlin, Celgene Cellular Therapeutics, Warren, NJ

Full text articles available: CLICK HERE>

Cell Medicine

Brazilian researchers find human menstrual blood-derived cells “feed” embryonic stem cells
Technique preserves the undifferentiated nature of hESCs destined for transplantation

Tampa, Fla. (May 28, 2014)—To be suitable for medical transplantation, one idea is that human embryonic stem cells (hESCs) need to remain “undifferentiated” i.e. they are not changing into other cell types. In determining the best way to culture hESCs so that they remain undifferentiated and also grow, proliferate and survive, researchers have used blood cell “feeder-layer” cultures using animal-derived feeder cells, often from mice [mouse embryonic fibroblasts (MEFs)]. This approach has, however, been associated with a variety of contamination problems, including pathogen and viral transmission.

To avoid contamination problems, a Brazilian research team has investigated the use of human menstrual blood-derived mesenchymal cells (MBMCs) as feeder layers and found that “MBMCs can replace animal-derived feeder systems in human embryonic stem cell culture systems and support their growth in an undifferentiated stage.”

The study will be published in a future issue of Cell Medicine, but is currently freely available on-line as an unedited early e-pub at: http://www.ingentaconnect.com/content/cog/cm/pre-prints/content-CM1019silvadosSantos

“Human embryonic stem cells present a continuous proliferation in an undifferentiated state, resulting in an unlimited amount of cells with the potential to differentiate toward any type of cell in the human body,” said study corresponding author Dr. Regina Coeli dos Santos Goldenberg of the Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro. “These characteristics make hESCs good candidates for cell based therapies.”

Feeder-layers for hESCs comprised of MEFs have been efficiently used for decades but, because of the clinical drawbacks, the authors subsequently experimented with human menstrual blood cells as a potential replacement for animal-derived feeder-layers, not only for negating the contamination issues, but also because human menstrual blood is so accessible. MBMCs are without ethical encumbrances and shortages, nor are they difficult to access—a problem with other human cells, such as umbilical cord blood cells, adult bone marrow cells or placenta cells.

“Menstrual blood is derived from uterine tissues,” explained the researchers. “These cells are widely available 12 times a year from women of child-bearing age. The cells are easily obtained, possess the capability of long-term proliferation and are clinically compatible with hESCs-derived cells.”

The researchers found that their culture system using MBMCs as a feeder-layer for hESCs are the “closest and more suitable alternative to animal-free conditions for growing hESCs” and a “good candidate for large-expansion of cells for clinical application.” They also found no difference in growth factor expression when comparing the use of growth factors in both the standard feeder system using animal cells and the feeder system they tested using hESCs.

“It is also noteworthy to highlight that our group reported the rapid and efficient generation of induced pluripotent stem cells (iPSCs) from MBMCs, indicating that these cells can be used as a model to study patient-specific disease and that in the future they might be used in clinical settings.”

“This study provides a new means of culturing hESCs without potential xenocontamination and after further study to confirm that there is no contamination of the ESCs with the feeder cells, this could prove to be a viable way to culture ESCs for clinical purposes” said Dr. Maria C. O. Rodrigues, at the Ribeirão Preto School of Medicine, University of Sao Paulo, Brazil and section editor for Cell Medicine.

Citation: Silva dos Santos, D.; Coelho de Oliveira, V. C.; Asensi, K. D.; Vairo, L.; Carvalho, A. B.; Campos de Carvalho, A. C.; Goldenberg, R. C. dos S. Human Menstrual Blood Derived Mesenchymal Cells As New Human Feederlayer System For Human Embryonic Stem Cells. Cell Med. Appeared or available online March 3, 2014.

Contact: Dr. Regina Coeli dos Santos Goldenberg, Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Av Carlos Chagas Filho 373 Bloco G-Sala G2-053, Rio de Janeiro, RJ 21941-902, Brazil. Tel: +55 21 2562 6559 Email: This e-mail address is being protected from spambots. You need JavaScript enabled to view it

The editorial offices for CELL MEDICINE are at the Center of Excellence for Aging and Brain Repair, College of Medicine, the University of South Florida. Contact: David Eve, Ph.D. at This e-mail address is being protected from spambots. You need JavaScript enabled to view it

News Release by Florida Science Communications www.sciencescribe.net

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Abstracts: 1,027
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